Cobalt in PDB 3tgh: GAP50 the Anchor in the Inner Membrane Complex of Plasmodium

All present enzymatic activity of GAP50 the Anchor in the Inner Membrane Complex of Plasmodium:
3.1.3.2;

The structure of GAP50 the Anchor in the Inner Membrane Complex of Plasmodium, PDB code: 3tgh was solved by J.Bosch, M.H.Paige, A.Vaidya, L.Bergman, W.G.J.Hol, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.44 / 1.70
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	57.648, 57.648, 210.625, 90.00, 90.00, 120.00
R / Rfree (%)	17.3 / 19.9

The binding sites of Cobalt atom in the GAP50 the Anchor in the Inner Membrane Complex of Plasmodium (pdb code 3tgh). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the GAP50 the Anchor in the Inner Membrane Complex of Plasmodium, PDB code: 3tgh:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the GAP50 the Anchor in the Inner Membrane Complex of Plasmodium


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of GAP50 the Anchor in the Inner Membrane Complex of Plasmodium within 5.0Å range: probe atom residue distance (Å) B Occ A:Co601 b:55.0 occ:1.00 O A:HOH504 2.2 47.9 1.0 NE2 A:HIS195 2.2 31.1 1.0 O A:HOH598 2.4 51.1 1.0 O A:HOH20 2.6 38.5 1.0 O A:HOH599 2.8 52.3 1.0 CE1 A:HIS195 3.2 31.0 1.0 CD2 A:HIS195 3.2 32.2 1.0 OE2 A:GLU196 3.7 57.7 1.0 O A:HOH437 3.9 39.2 1.0 OH A:TYR235 4.0 41.1 1.0 ND1 A:HIS195 4.3 26.7 1.0 CG A:HIS195 4.4 28.8 1.0 O A:HOH600 4.4 67.3 1.0 NE1 A:TRP199 4.7 30.2 1.0 CD1 A:TRP199 4.7 29.8 1.0 CD A:GLU196 4.7 51.3 1.0

Cobalt binding site 2 out of 2 in the GAP50 the Anchor in the Inner Membrane Complex of Plasmodium


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of GAP50 the Anchor in the Inner Membrane Complex of Plasmodium within 5.0Å range: probe atom residue distance (Å) B Occ A:Co21 b:50.1 occ:1.00 O A:HOH606 2.0 38.5 1.0 NE2 A:HIS256 2.2 28.5 1.0 O A:HOH604 2.3 33.3 1.0 O A:HOH605 2.4 30.6 1.0 O A:HOH607 2.5 33.8 1.0 CE1 A:HIS256 3.1 33.0 1.0 CD2 A:HIS256 3.2 28.8 1.0 O A:HOH370 4.0 24.8 1.0 O A:HOH608 4.1 67.0 1.0 OE1 A:GLN222 4.2 27.7 1.0 ND1 A:HIS256 4.2 30.9 1.0 CG A:HIS256 4.3 26.2 1.0 O A:HOH397 4.5 25.3 1.0 CB A:SER230 4.6 29.6 1.0

J.Bosch, M.H.Paige, A.B.Vaidya, L.W.Bergman, W.G.Hol. Crystal Structure of GAP50, the Anchor of the Invasion Machinery in the Inner Membrane Complex of Plasmodium Falciparum. J.Struct.Biol. V. 178 61 2012.
ISSN: ISSN 1047-8477
PubMed: 22387043
DOI: 10.1016/J.JSB.2012.02.009