Cobalt in PDB 3w45: Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1

All present enzymatic activity of Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1:
3.1.3.3;

The structure of Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1, PDB code: 3w45 was solved by M.Makino, A.H.Teh, S.Baba, N.Shimizu, M.Yamamoto, T.Kumasaka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.67 / 1.70
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	33.108, 41.614, 68.806, 80.81, 89.95, 71.08
R / Rfree (%)	17 / 20.2

The binding sites of Cobalt atom in the Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1 (pdb code 3w45). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1, PDB code: 3w45:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:17.8 occ:0.69 OD1 A:ASP156 2.0 13.9 1.0 O A:HOH353 2.0 14.6 1.0 OD2 A:ASP44 2.1 16.0 1.0 O A:HOH352 2.2 15.0 1.0 OD2 A:ASP189 2.2 16.3 1.0 O A:HOH351 2.2 16.1 1.0 CG A:ASP156 3.0 14.5 1.0 CG A:ASP44 3.1 15.4 1.0 CG A:ASP189 3.2 17.9 1.0 OD2 A:ASP156 3.3 15.0 1.0 OD1 A:ASP44 3.5 12.5 1.0 OD1 A:ASP189 3.5 17.2 1.0 NZ B:LYS147 4.0 15.2 1.0 O A:HOH358 4.0 12.7 1.0 O A:HOH356 4.0 17.3 1.0 O B:HOH500 4.1 18.0 1.0 OD1 A:ASP27 4.2 15.4 1.0 O A:HOH361 4.2 15.0 1.0 N A:GLY157 4.2 17.3 1.0 O A:ASP190 4.3 18.0 1.0 CB A:ASP156 4.4 16.1 1.0 CB A:ASP44 4.4 13.7 1.0 N A:ASP156 4.5 15.5 1.0 CB A:ASP189 4.5 18.6 1.0 C A:ASP156 4.6 17.6 1.0 CA A:ASP156 4.7 16.6 1.0 CE B:LYS147 4.8 13.4 1.0 CB A:THR155 4.8 13.7 1.0 O A:HOH301 4.9 25.3 1.0 CA A:GLY157 4.9 18.6 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Rsbx in Complex with Cobalt in Space Group P1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Co201 b:20.1 occ:0.81 O B:HOH353 2.0 14.1 1.0 O B:HOH351 2.1 15.2 1.0 OD2 B:ASP44 2.1 14.5 1.0 OD1 B:ASP156 2.2 15.2 1.0 O B:HOH352 2.2 15.3 1.0 OD2 B:ASP189 2.2 16.2 1.0 CG B:ASP44 3.0 15.5 1.0 CG B:ASP156 3.1 16.4 1.0 CG B:ASP189 3.1 16.4 1.0 OD1 B:ASP44 3.4 14.9 1.0 OD1 B:ASP189 3.4 15.6 1.0 OD2 B:ASP156 3.4 17.1 1.0 O B:HOH354 4.0 14.9 1.0 O B:HOH301 4.0 20.3 1.0 O B:HOH420 4.1 15.4 1.0 O B:HOH422 4.1 17.7 1.0 OD1 B:ASP27 4.1 16.1 1.0 O B:ASP190 4.4 15.9 1.0 N B:GLY157 4.4 17.3 1.0 CB B:ASP44 4.4 14.0 1.0 CB B:ASP189 4.5 15.8 1.0 CB B:ASP156 4.5 15.6 1.0 N B:ASP156 4.6 16.5 1.0 C B:ASP156 4.8 17.7 1.0 O B:HOH355 4.8 29.3 1.0 CA B:ASP156 4.8 17.1 1.0 CB B:THR155 4.9 13.7 1.0

M.Makino, A.H.Teh, T.Hoshino, S.Baba, N.Shimizu, M.Yamamoto, T.Kumasaka. Structural Basis For Di-Metal Coordination and Negative Feedback in General Stress Response By Rsbx Phosphatase From Bacillus Subtilis To Be Published.