Cobalt in PDB 4a8l: Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission

All present enzymatic activity of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission:
5.3.1.5;

The structure of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8l was solved by K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.87 / 1.35
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.560, 98.420, 102.130, 90.00, 90.00, 90.00
R / Rfree (%)	11.007 / 13.676

The binding sites of Cobalt atom in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission (pdb code 4a8l). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8l:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1389 b:13.2 occ:1.00 OE1 A:GLU217 2.1 15.7 1.0 OD2 A:ASP287 2.1 11.3 1.0 OE2 A:GLU181 2.1 16.1 1.0 OD2 A:ASP245 2.3 10.5 1.0 O2 A:EDO1388 2.4 11.9 1.0 O A:HOH2277 2.4 24.8 1.0 CD A:GLU181 3.1 11.2 1.0 CG A:ASP287 3.2 7.2 1.0 C2 A:EDO1388 3.3 13.8 1.0 CD A:GLU217 3.3 8.0 1.0 OE1 A:GLU181 3.4 10.8 1.0 CG A:ASP245 3.5 7.2 1.0 CB A:ASP287 3.6 6.4 1.0 O A:HOH2331 3.9 13.4 1.0 O A:HOH2301 3.9 17.0 1.0 CB A:ASP245 4.0 5.9 1.0 CE1 A:HIS220 4.1 15.1 1.0 CB A:GLU217 4.2 5.9 1.0 CG A:GLU217 4.2 6.0 1.0 OE2 A:GLU217 4.3 13.5 1.0 OD1 A:ASP287 4.3 7.6 1.0 CG A:GLU181 4.4 8.2 1.0 OD1 A:ASP245 4.4 9.2 1.0 NE2 A:HIS220 4.5 12.8 1.0 C1 A:EDO1388 4.7 9.5 1.0 ND2 A:ASN215 4.8 10.6 1.0 ND1 A:HIS220 4.8 9.4 1.0 CO A:CO1390 4.9 21.8 1.0

Cobalt binding site 2 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1390 b:21.8 occ:1.00 OE2 A:GLU217 2.0 13.5 1.0 O A:HOH2301 2.1 17.0 1.0 OD1 A:ASP255 2.1 18.4 1.0 OD2 A:ASP255 2.3 18.4 1.0 OD1 A:ASP257 2.5 19.3 1.0 CG A:ASP255 2.7 15.7 1.0 NE2 A:HIS220 2.8 12.8 1.0 CD A:GLU217 3.0 8.0 1.0 CD2 A:HIS220 3.3 9.4 1.0 O A:HOH2281 3.3 24.1 1.0 OE1 A:GLU217 3.5 15.7 1.0 CG A:ASP257 3.5 14.4 1.0 OD2 A:ASP257 3.7 18.7 1.0 CE1 A:HIS220 3.9 15.1 1.0 ND2 A:ASN247 3.9 6.9 1.0 CB A:ASP255 4.1 14.5 1.0 O A:HOH2333 4.2 13.5 1.0 O A:HOH2277 4.4 24.8 1.0 CG A:GLU217 4.4 6.0 1.0 CG A:HIS220 4.5 6.8 1.0 NZ A:LYS183 4.7 11.0 1.0 OD2 A:ASP287 4.8 11.3 1.0 CE A:LYS183 4.8 7.3 1.0 ND1 A:HIS220 4.8 9.4 1.0 CB A:ASP257 4.8 9.9 1.0 CA A:ASP255 4.9 8.9 1.0 CO A:CO1389 4.9 13.2 1.0

K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes. Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission To Be Published.