Cobalt in PDB 5ol5: Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein

All present enzymatic activity of Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein:
2.7.7.7;

The structure of Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein, PDB code: 5ol5 was solved by L.M.Kick, S.Schneider, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.22 / 2.33
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	60.120, 92.129, 110.448, 90.00, 90.00, 90.00
R / Rfree (%)	23.1 / 27.6

The binding sites of Cobalt atom in the Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein (pdb code 5ol5). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein, PDB code: 5ol5:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:42.9 occ:1.00 NE2 A:HIS24 2.0 64.7 1.0 NE2 D:HIS40 2.1 56.4 1.0 O A:HOH328 2.1 34.1 1.0 CE1 A:HIS24 2.8 68.7 1.0 CE1 D:HIS40 3.0 59.9 1.0 CD2 D:HIS40 3.0 55.5 1.0 CD2 A:HIS24 3.1 65.2 1.0 ND1 A:HIS24 4.0 66.7 1.0 ND1 D:HIS40 4.1 56.8 1.0 CG A:HIS24 4.2 62.5 1.0 CG D:HIS40 4.2 52.0 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of An Inactivated Ssp Siclopps Intein with Cfahpq Extein within 5.0Å range: probe atom residue distance (Å) B Occ D:Co201 b:48.4 occ:1.00 NE2 D:HIS35 2.0 77.6 1.0 CD2 D:HIS35 2.9 76.0 1.0 CE1 D:HIS35 3.0 81.3 1.0 CG D:HIS35 4.0 72.2 1.0 ND1 D:HIS35 4.0 78.0 1.0 CB D:ALA34 4.3 45.3 1.0 CB D:ALA32 4.8 56.3 1.0

L.M.Kick, S.Harteis, M.F.Koch, S.Schneider. Mechanistic Insights Into Cyclic Peptide Generation By Dnae Split-Inteins Through Quantitative and Structural Investigation. Chembiochem V. 18 2242 2017.
ISSN: ESSN 1439-7633
PubMed: 28914478
DOI: 10.1002/CBIC.201700503