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Cobalt in PDB 1egn: Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant

Enzymatic activity of Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant

All present enzymatic activity of Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant:
3.2.1.91;

Protein crystallography data

The structure of Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant, PDB code: 1egn was solved by J.Stahlberg, M.Harris, T.A.Jones, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.60
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 83.110, 83.340, 110.610, 90.00, 90.00, 90.00
R / Rfree (%) 24 / 27

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant (pdb code 1egn). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant, PDB code: 1egn:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 1egn

Go back to Cobalt Binding Sites List in 1egn
Cobalt binding site 1 out of 2 in the Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co470

b:7.6
occ:0.50
OE2 A:GLU325 2.2 6.8 1.0
OE1 A:GLU295 2.3 7.4 1.0
OE2 A:GLU295 2.3 7.9 1.0
CD A:GLU295 2.6 7.8 1.0
CD A:GLU325 3.2 7.7 1.0
OE1 A:GLU325 3.5 7.7 1.0
O A:HOH742 3.8 13.0 1.0
ND2 A:ASN301 4.0 6.5 1.0
CG A:GLU295 4.1 7.7 1.0
O A:HOH597 4.2 9.7 1.0
CG A:GLU325 4.5 7.7 1.0
OG A:SER297 4.7 9.6 1.0
CB A:ALA299 4.7 7.7 1.0
O A:HOH599 4.9 10.9 1.0
CB A:GLU295 4.9 7.8 1.0

Cobalt binding site 2 out of 2 in 1egn

Go back to Cobalt Binding Sites List in 1egn
Cobalt binding site 2 out of 2 in the Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Cellobiohydrolase CEL7A (E223S, A224H, L225V, T226A, D262G) Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co471

b:28.2
occ:1.00
NE2 A:HIS206 2.2 14.6 1.0
OE1 A:GLU239 2.5 20.8 1.0
CE1 A:HIS206 3.1 14.8 1.0
CD A:GLU239 3.2 19.9 1.0
OE2 A:GLU239 3.2 21.5 1.0
CD2 A:HIS206 3.3 14.5 1.0
ND1 A:HIS206 4.2 14.4 1.0
CG A:HIS206 4.3 14.0 1.0
CG A:GLU239 4.7 19.0 1.0

Reference:

D.Becker, C.Braet, H.Brumer Iii, M.Claeyssens, C.Divne, B.R.Fagerstrom, M.Harris, T.A.Jones, G.J.Kleywegt, A.Koivula, S.Mahdi, K.Piens, M.L.Sinnott, J.Stahlberg, T.T.Teeri, M.Underwood, G.Wohlfahrt. Engineering of A Glycosidase Family 7 Cellobiohydrolase to More Alkaline pH Optimum: the pH Behaviour of Trichoderma Reesei CEL7A and Its E223S/ A224H/L225V/T226A/D262G Mutant. Biochem.J. V. 356 19 2001.
ISSN: ISSN 0264-6021
PubMed: 11336632
DOI: 10.1042/0264-6021:3560019
Page generated: Sun Dec 13 10:34:39 2020

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