Cobalt in PDB 1fa6: Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli

All present enzymatic activity of Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli:
4.4.1.5;

The structure of Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli, PDB code: 1fa6 was solved by M.M.He, S.L.Clugston, J.F.Honek, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.090, 56.570, 46.750, 90.00, 95.54, 90.00
R / Rfree (%)	16.9 / 25.4

The binding sites of Cobalt atom in the Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli (pdb code 1fa6). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli, PDB code: 1fa6:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1200 b:26.2 occ:1.00 OE1 A:GLU56 2.1 13.4 1.0 OE1 B:GLU122 2.1 18.8 1.0 O A:HOH1292 2.2 15.8 1.0 NE2 A:HIS5 2.2 15.5 1.0 NE2 B:HIS74 2.3 12.5 1.0 O B:HOH217 2.4 21.4 1.0 CE1 A:HIS5 3.1 16.7 1.0 CD A:GLU56 3.1 30.2 1.0 CD B:GLU122 3.2 28.3 1.0 CE1 B:HIS74 3.2 11.6 1.0 CD2 A:HIS5 3.3 17.1 1.0 CD2 B:HIS74 3.4 17.0 1.0 OE2 A:GLU56 3.5 18.5 1.0 O B:HOH254 3.6 49.8 1.0 OE2 B:GLU122 3.7 29.1 1.0 O A:HOH1247 4.1 29.1 1.0 ND1 A:HIS5 4.3 19.1 1.0 CB B:ALA76 4.4 14.2 1.0 CG A:HIS5 4.4 18.3 1.0 ND1 B:HIS74 4.4 15.7 1.0 CB A:MET7 4.5 13.9 1.0 CG B:GLU122 4.5 14.3 1.0 CG A:GLU56 4.5 16.3 1.0 CG B:HIS74 4.5 15.7 1.0 CB B:GLU122 4.6 13.5 1.0 CB A:GLU56 4.7 14.4 1.0 CG A:MET7 4.8 14.0 1.0 O A:HOH1295 5.0 39.2 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of the Co(II)-Bound Glyoxalase I of Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1201 b:24.9 occ:1.00 O B:HOH267 2.0 15.2 1.0 OE1 B:GLU56 2.2 15.2 1.0 NE2 B:HIS5 2.2 11.1 1.0 OE1 A:GLU122 2.3 43.1 1.0 O A:HOH1267 2.3 26.3 1.0 NE2 A:HIS74 2.4 11.8 1.0 CE1 B:HIS5 3.1 10.5 1.0 CD A:GLU122 3.1 37.1 1.0 CE1 A:HIS74 3.1 11.3 1.0 CD B:GLU56 3.2 22.5 1.0 CD2 B:HIS5 3.3 14.2 1.0 OE2 A:GLU122 3.4 19.4 1.0 CD2 A:HIS74 3.5 16.1 1.0 OE2 B:GLU56 3.7 26.2 1.0 O B:HOH228 4.1 29.3 1.0 ND1 B:HIS5 4.3 13.6 1.0 CB A:ALA76 4.3 14.5 1.0 CG B:HIS5 4.3 14.4 1.0 ND1 A:HIS74 4.4 12.9 1.0 CG A:GLU122 4.5 18.8 1.0 CG B:GLU56 4.5 10.5 1.0 CG A:HIS74 4.5 12.5 1.0 CB B:MET7 4.6 12.7 1.0 CB A:GLU122 4.7 13.8 1.0 CB B:GLU56 4.8 12.3 1.0 O B:HOH178 4.8 29.5 1.0 CG B:MET7 4.9 13.7 1.0

M.M.He, S.L.Clugston, J.F.Honek, B.W.Matthews. Determination of the Structure of Escherichia Coli Glyoxalase I Suggests A Structural Basis For Differential Metal Activation. Biochemistry V. 39 8719 2000.
ISSN: ISSN 0006-2960
PubMed: 10913283
DOI: 10.1021/BI000856G